By Richard A. McFeely

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Extra resources for Advances in Veterinary Science and Comparative Medicine: Domestic Animal Cytogenetics (Advances in Veterinary Medicine) (v. 34)

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L. (1962). Symposium on sex chromatin: The sex chromatin: Its discovery and variation in the animal kingdom. Acta Cytol. 6, 1-12. Moorhead, P. , Nowell, P. C , Mellman, W. , Batipps, D. , and Hungerford, D. A. (1960). Chromosome preparations of leucocytes cultured from peripheral blood. Exp. Cell Res. 20, 613-616. Nowell, p. C. (1960a). Differentiation of human leukemic leucocytes in tissue culture. Exp. Cell Res. 19, 267-277. Nowell, p. C. (1960b). Phytohemagglutinin: An initiator of mitosis in cultures of normal human leukocytes.

The BrdU causes differential contraction along the chromosome and subse­ quent staining with acridine orange results in fluorescent bands (Du­ trillaux et al, 1973). A modification of this staining technique is the fluorescent-plus-Giemsa (FPG) technique of Perry and Wolff (1974). This involves staining with 33258 Hoechst, exposing the preparation to exciting light, and then counterstaining with Giemsa. Bands of light and dark Giemsa staining are produced, correlating to the positive and negative fluorescence of the fluorochrome.

The BrdU causes differential contraction along the chromosome and subse­ quent staining with acridine orange results in fluorescent bands (Du­ trillaux et al, 1973). A modification of this staining technique is the fluorescent-plus-Giemsa (FPG) technique of Perry and Wolff (1974). This involves staining with 33258 Hoechst, exposing the preparation to exciting light, and then counterstaining with Giemsa. Bands of light and dark Giemsa staining are produced, correlating to the positive and negative fluorescence of the fluorochrome.

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Advances in Veterinary Science and Comparative Medicine: by Richard A. McFeely
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